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E2F6 polyclonal antibody

货号: BS8510

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8510

Product Name :E2F6 polyclonal antibody

Swiss-Prot :O75461

Host :Rabbit

Reactivity :Human, Mouse, Rat, Bovine, Pig

Applications :WB IHC IP CHIP

Application_all :WB: 1:500~1:1000 IHC: 1:100~1:200 IP: 1:10~1:100 CHIP: 1:100~1:500

Background :The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcriptional regulators including E2F-2, E2F-3, E2F-4, E2F-5 and E2F-6, each of which forms heterodimers with a second protein, DP-1, forming an “active” E2F transcriptional regulatory complex.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :E2F6 polyclonal antibody detects endogenous levels of E2F6 protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,

Alternative Name :Transcription factor E2F6; E2F-6

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F6.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of E2F6 expression in PC12 (A), A2780 (B), HEK293T (C), HCT116 (D) whole cell lysates.

Immunohistochemical analysis of E2F6 staining in human brain formalin fixed paraffin embedded tissue section.

ChIP analysis of Mouse ES cell nuclear lysate, incubated for 16 hours at 4°C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Tuba3. Negative Control: Beta-Actin. Detection step: Real-time PCR.