产品类别

联系我们

电话:18013895086

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKA C-alpha (PRKACA) polyclonal antibody

货号: BS79572

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS79572

Product Name :PKA C-alpha (PRKACA) polyclonal antibody

Swiss-Prot :P17612

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IHC IF/ICC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:100 IF/ICC: 1:50~1:100 IP: 1:10~1:50

Background :This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKA C-alpha (PRKACA) polyclonal antibody detects endogenous levels of PKA C-alpha (PRKACA) protein.

BiowMW :41KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha

Immunogen :A synthetic peptide of human PKA C-alpha (PRKACA)(NP_002721.1).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of PKA C-alpha (PRKACA) expression in K562 (A), C6 (B), NIH3T3 (C), Hela (D) whole cell lysates.

Immunohistochemical analysis of PKA C-alpha (PRKACA) staining in human colon cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of PKA C-alpha (PRKACA) staining in HeLa cells.

免疫沉淀（IP）