产品类别

联系我们

电话:18013895086

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

E2F1 polyclonal antibody

货号: BS66106

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS66106

Product Name :E2F1 polyclonal antibody

Swiss-Prot :Q01094

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF/ICC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:100 IF/ICC: 1:50~1:100 IP: 1:10~1:50

Background :The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :E2F1 polyclonal antibody detects endogenous levels of E2F1 protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling，Cell Cycle Control G1 S Checkpoin

Alternative Name :RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1

Immunogen :Synthetic peptide, corresponding to Human E2F1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of E2F1 expression in Molt4 (A), C6 (B), NIH3T3 (C) whole cell lysates.

Immunohistochemical analysis of E2F1 staining in human tonsil formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of E2F1 staining in HeLa cells.