Product Name :Strep II Tag Mouse monoclonal antibody
Application_all :WB: 1:5000~1:10000
IF: 1:200
Background :Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II.
Product :Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :Affinity-chromatography
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Strep Ⅱtag mouse monoclonal antibody detects endogenous levels of internal, C-terminal, and N-terminal Strep Ⅱ- tagged proteins.
Figure1 :1ug Strep II fusion protein+ Primary antibody dilution at
1.1:5000
2.1:10000
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :Strep-Tag II; Strep II
Immunogen :Synthetic peptide Strep II Tag.
Modification :Unmodification