Product Name :Flag-Tag mouse monoclonal antibody
Applications :WB IHC IF IP
Application_all :WB: 1:20000~1:100000
IHC/IF: 1:50~1:200
IP: 1:50~1:200
Background :Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from cell culture supernatant by protein A+G and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Flag-Tag mAb detects over-expressed or recombinant proteins containing the Flag epitope tag.
Figure1 :Western blot (WB) analysis of Flag-Tag mAb at 1:100000 dillution
Lane1:HEK293F whole cell lysate ,untransfected
Lane2:HEK293F whole cell lysate , transfected (M-Flag)
Lane3:HEK293F whole cell lysate , transfected (N-Flag)
Lane4:HEK293F whole cell lysate , transfected (C-Flag)
Figure3 :Immunofluorescent analysis of Flag-Tag mAb staining in transfected HEK293F cells.
Figure4 :Immunoprecipitation of transfected HEK293F using Flag-Tag mAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using Flag-Tag mAb.“↑”(supernatant); “↓”(deposition)
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK
Immunogen :Synthetic peptide Flag-tag.
