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NANOG monoclonal antibody

货号: MB67211

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB67211

Product Name :NANOG monoclonal antibody

Swiss-Prot :Q9H9S0

Host :Mouse

Reactivity :Human

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/50 - 1/200)

Background :Nanog is a homeodomain-containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells. Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4. Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc, and LIN28.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of NANOG protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ESC Pluripotency and Differentiation Signaling Pathway

Alternative Name :Homeobox protein NANOG; Homeobox transcription factor Nanog; hNanog

Immunogen :Recombinant fusion protein of human NANOG. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of NANOG expression in Caco2 (A), MCF7 (B) whole cell lysates.

Immunohistochemical analysis of NANOG staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.