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PRAS40 monoclonal antibody

货号: MB67171

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB67171

Product Name :PRAS40 monoclonal antibody

Swiss-Prot :Q96B36

Host :Mouse

Reactivity :Human, Mouse

Applications :WB

Application_all :WB (1/500 - 1/2000)

Background :Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity. PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1. mTORC1 in turn phosphorylates PRAS40 at Ser183.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PRAS40 protein.

BiowMW :~ 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling, PI3K AKT signaling, T Cell Receptor Signaling, Translational Contral elF4 and p70 S6 Kinase, mTOR Signaling

Alternative Name :PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate

Immunogen :Recombinant fusion protein of human PRAS40. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PRAS40 expression in 293 (A), Hela (B), MCF7 (C), NIH3T3 (D) whole cell lysates.