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RAPTOR monoclonal antibody

货号: MB67149

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB67149

Product Name :RAPTOR monoclonal antibody

Swiss-Prot :Q8N122

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/1000 - 1/2000), IHC (1/50 - 1/200)

Background :The regulatory associated protein of mTOR (Raptor) was identified as an mTOR binding partner that mediates mTOR signaling to downstream targets. Raptor binds to mTOR substrates, including 4E-BP1 and p70 S6 kinase, through their TOR signaling (TOS) motifs and is required for mTOR-mediated phosphorylation of these substrates. Binding of the FKBP12-rapamycin complex to mTOR inhibits the mTOR-raptor interaction, suggesting a mechanism for rapamycin's specific inhibition of mTOR signaling. This mTOR-raptor interaction and its regulation by nutrients and/or rapamycin is dependent on a protein called GβL. GβL is also part of the rapamycin-insensitive complex between mTOR and rictor (rapamycin-insensitive companion of mTOR), and may mediate rictor-mTOR signaling to downstream targets including PKCα. Furthermore, the rictor-mTOR complex has been identified as the previously elusive PDK2 responsible for the phosphorylation of Akt/PKB on Ser473, facilitating phosphorylation of Akt/PKB on Thr308 by PDK1 and required for the full activation of Akt/PKB. Recently raptor has been identified as a direct substrate of the AMP-activated protein kinase (AMPK). AMPK phosphorylates raptor on Ser722/Ser792. This phosphorylation is essential for inhibition of the raptor-containing mTOR complex 1 (mTORC1) and induces cell cycle arrest when cells are stressed for energy. These findings suggest that raptor is a critical switch that correlates cell cycle progression with energy status.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of RAPTOR protein.

BiowMW :~ 150 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :AMPK Signaling Pathway，Autophagy Signaling，T cell receptor signaling，MTOR signaling

Alternative Name :KIAA1303; RAPTOR; Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR)-scaffold protein

Immunogen :Recombinant fusion protein of human RAPTOR. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of RAPTOR expression in Hela (A), MCF7 (B), C2C12 (C), NIH3T3 (D), C6 (E) whole cell lysates.

Immunohistochemical analysis of RAPTOR staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.