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FAT1 monoclonal antibody

货号: MB67100

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB67100

Product Name :FAT1 monoclonal antibody

Swiss-Prot :Q14517

Host :Mouse

Reactivity :Human

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :FAT1 is a member of the FAT atypical cadherin (FAT) subfamily of cadherin proteins. FAT1 is a single-pass transmembrane protein, first identified in a screen for tumor suppressor proteins in Drosophila. FAT1 is expressed primarily in epithelial cells, where it plays a prominent role in regulating cell growth and migration, in large part through the regulation of cell-cell adhesion dynamics. The intracellular cytoplasmic tail of FAT1 contains multiple functional motif/domains that regulate FAT1 functions, including a proline rich EVH1 binding motif that regulates actin cytoskeleton components (e.g., Ena/VASP proteins) at both cell-cell contact points and leading edges of migrating cells . FAT1 appears to play a role in linking cell adhesion events to intracellular signaling pathways. For example, FAT1 was capable of inhibiting the nuclear translocation of β-catenin through its cytoplasmic FC1 domain interaction with β-catenin, and activating the Hippo signaling pathway, suppressing YAP signaling by its N-terminal cytoplasmic region interaction with MST1 . Research studies have revealed that the tumor suppressor functions identified in Drosophila are conserved in vertebrate FAT1 homologs. For example, studies in human cancer cells showed that loss-of-function mutations in the gene encoding FAT1 promoted a hybrid epithelial-to-mesenchymal transition, which further enabled the development of cancer drug resistance. Notably, studies have also revealed an oncogenic function for FAT1 in some contexts.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of FAT1 protein.

BiowMW :~ 506 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CDHF7; FAT; Protocadherin Fat 1; Cadherin family member 7; Cadherin-related tumor suppressor homolog; Protein fat homolog

Immunogen :Recombinant fusion protein of human FAT1. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of FAT1 expression in recombinant protein (A) whole cell lysates.

免疫沉淀（IP）