Product Name :Rac 1 monoclonal antibody
Applications :WB, IHC, FC
Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200), FC (1/10 - 1/50)
Background :Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development . GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state .
Product :Mouse IgG2b. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :This antibody is purified through a protein G column.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of Rac 1 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics,Adherens Junction signaling,ErbB HER Signaling,MAPK Erk in Growth and Differentiation Pathway,Regulation of Microtubule Dynamics,SAPK JNK Signaling Cascades,T cell receptor signaling,TGFβ Smad signaling,Wntβ catenin signaling.
Alternative Name :TC25; Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1
Immunogen :KLH-conjugated synthetic peptide of human Rac 1. The exact sequence is proprietary.
Modification :Unmodification
