Product Name :RhoA monoclonal antibody
Reactivity :Human, Mouse, Rat
Application_all :WB (1/1000 - 1/8000)
Background :Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) . RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules .Functions of RhoA, the most highly studied of these three, include regulation of actomyosin contractility , cytokinesis , focal adhesion assembly and cell polarity .
Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :This antibody is purified through a protein G column.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of RhoA protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics,Adherens Junction signaling,Regulation of Microtubule Dynamics,SAPK JNK Signaling Cascades,TGFβ Smad signaling.
Alternative Name :ARH12; ARHA; RHO12; Transforming protein RhoA; Rho cDNA clone 12; h12
Immunogen :Recombinant fusion protein of human RhoA. The exact sequence is proprietary.
Modification :Unmodification
