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p27 Kip1 monoclonal antibody

货号: MB66990

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66990

Product Name :p27 Kip1 monoclonal antibody

Swiss-Prot :P46527

Host :Mouse

Reactivity :Human

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background :p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors. Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes. Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators. Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway .

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of p27 Kip1 protein.

BiowMW :~ 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling.

Alternative Name :KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1

Immunogen :Recombinant fusion protein of human p27 Kip1. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of p27 Kip1 expression in MCF7 (A), U266 (B) whole cell lysates.

Immunohistochemical analysis of p27 Kip1 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.