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EIF4B monoclonal antibody

货号: MB66938

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66938

Product Name :EIF4B monoclonal antibody

Swiss-Prot :P23588

Host :Mouse

Reactivity :Human

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :Eukaryotic initiation factor 4B is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity . Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation . Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 . Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens , and the phosphorylation of this site is dependent on MEK and mTOR activity . This phosphorylation is shown to be essential for the translational activity of eIF4B.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of EIF4B protein.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral Regulation of elF2,Translational Contral elF4 and p70 S6 Kinase

Alternative Name :Eukaryotic translation initiation factor 4B; eIF-4B

Immunogen :Recombinant fusion protein of human EIF4B. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of EIF4B expression in A431 (A), Hela (B) whole cell lysates.