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c-CBL monoclonal antibody

货号: MB66934

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB66934

Product Name :c-CBL monoclonal antibody

Swiss-Prot :P22681

Host :Mouse

Reactivity :Human

Applications :WB

Application_all :WB (1/500 - 1/1000)

Background :The c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells . c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol-3 kinase, Crk, and 14-3-3 proteins. c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. The RING finger motif recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for the ubiquitin ligase activity of c-Cbl and downregulation of RTKs . Research studies have indicated that in human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner. Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn.

Product :Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of c-CBL protein.

BiowMW :~ 120 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ErbB HER Signaling,Insulin &Glucose Signaling,T cell receptor signaling

Alternative Name :CBL2; RNF55; E3 ubiquitin-protein ligase CBL; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human c-CBL. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of c-CBL expression in K562 (A), Jurkat (B), Raji (C), SKBR3 (D) whole cell lysates.