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PGP9.5 monoclonal antibody

货号: MB66855

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66855

Product Name :PGP9.5 monoclonal antibody

Swiss-Prot :P09936

Host :Mouse

Reactivity :Human, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Background :Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.Also binds to free monoubiquitin and may prevent its degradation in lysosomes.The homodimer may have ATP-independent ubiquitin ligase activity.

Product :Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of PGP9.5 protein.

BiowMW :~ 25 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PGP9.5. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of PGP9.5 expression in 293T (A), NCIH1299 (B), PC12 (C) whole cell lysates.

Immunohistochemical analysis of PGP9.5 staining in human astroglioma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.