Product Name :HCK monoclonal antibody
Application_all :WB (1/1000 - 1/2000), IF/ICC (1/10 - 1/50)
Background :Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS.
Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Purification&Purity :This antibody is purified through a protein G column.
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Recognizes endogenous levels of HCK protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics，Regulation of Microtubule Dynamics，Tumor Angiogenesis，Death Receptor Signaling，MAPK Erk in Growth and Differentiation Pathway，Jak Stat ：IL-6 Receptor Signaling，Insulin &Glucose Signaling，Regulation of Microtubule Dynamics，PI3K AKT signaling，T cell receptor signaling，Signaling Pathways Activating p38 MAP Kinase，Wntβ catenin signaling
Alternative Name :Tyrosine-protein kinase HCK; Hematopoietic cell kinase; Hemopoietic cell kinase; p59-HCK/p60-HCK; p59Hck; p61Hck
Immunogen :Recombinant fusion protein of human HCK. The exact sequence is proprietary.
Modification :Unmodification
