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c-Met monoclonal antibody

货号: MB66843

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66843

Product Name :c-Met monoclonal antibody

Swiss-Prot :P08581

Host :Mouse

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Background :Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis.

Product :Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of c-Met protein.

BiowMW :~ 140 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Hepatocyte growth factor receptor; HGF receptor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met

Immunogen :Recombinant fusion protein of human c-Met. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of c-Met expression in Hela (A), HepG2 (B), COS7 (C) whole cell lysates.

Immunohistochemical analysis of c-Met staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.