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SOD2 monoclonal antibody

货号: MB66793

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66793

Product Name :SOD2 monoclonal antibody

Swiss-Prot :P04179

Host :Mouse

Reactivity :Human, Mouse

Applications :WB, IHC, FC

Application_all :WB (1/500 - 1/1000), IHC (1/50 - 1/200), FC (1/10 - 1/50)

Background :The superoxide dismutase family is composed of three metalloenzymes (SOD-1, SOD-2 and SOD-3) that catalyze the oxido-reduction of reactive oxygen species (ROS) such as superoxide anion. The SOD-2 precursor is a 222 amino acid protein that is encoded by nuclear chromatin, synthesized in the cytosol and imported posttranslationally into the mitochondrial matrix. Unlike SOD-1, which is a homodimeric cytosolic Cu-Zn enzyme, SOD-2 is a homotetrameric manganese enzyme (also known as MnSOD) that functions in the mitochondrion. ROS are implicated in a wide range of degenerative processes, including Alzheimer’s disease, Parkinson’s disease and ischemic heart disease. Homozygous mutant mice, which lack SOD-2, exhibit dilated cardiomyopathy, accumulation of lipid in liver and skeletal muscle, metabolic acidosis, oxidative DNA damage and respiratory chain deficiencies in heart and skeletal muscle. Polymorphisms in the SOD-2 gene have also been implicated in nonfamilial, idiopathic, dilated cardiomyopathy in humans.

Product :Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :This antibody is purified through a protein G column.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of SOD2 protein.

BiowMW :~ 26 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Superoxide dismutase [Mn], mitochondrial

Immunogen :Recombinant fusion protein of human SOD2. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of SOD2 expression in human brain (A), Hela (B), MCF7 (C), NIH3T3 (D), SHSY5Y (E) whole cell lysates.

Immunohistochemical analysis of SOD2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-oxidant and Anti-inflammatory Effects of Ethanol Extract from Polygala sibirica L. var megalopha Fr. on Lipopolysaccharide-Stimulated RAW264.7 Cells

JournalChinese Journal of Integrative Medicine

ApplicationWB

IF2.9

SpeciesMouse

PMID37434032