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EEF2 monoclonal antibody

货号: MB66520

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66520

Product Name :EEF2 monoclonal antibody

Swiss-Prot :P13639

Host :Mouse

Reactivity :Human

Applications :WB, IF/ICC, ChIP

Application_all :WB (1/500 - 1/1000), IF/ICC (1/50 - 1/100), ChIP (1/10 - 1/50)

Background :Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tRNA from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kinase inhibits its activity. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The inactive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR signaling and may involve modulation of the activity of the PP-2A or the eEF2 kinase or both.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :The antibody was purified by immunogen affinity chromatography.

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of EEF2 protein.

BiowMW :~ 95 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EF2; Elongation factor 2; EF-2

Immunogen :Purified recombinant human eEF2 protein fragments expressed in E.coli.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of EEF2 expression in HL60 (A), Jurkat (B), SHSY5Y (C), U20S (D), Hela (E) whole cell lysates.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.