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alpha Smooth Muscle Actin monoclonal antibody

货号: MB66146

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB66146

Product Name :alpha Smooth Muscle Actin monoclonal antibody

Swiss-Prot :P62736

Host :Mouse

Reactivity :Human,Mouse,Rat

Applications :WB IHC FC

Application_all :WB: 1:500~1:1000 IHC: 1:100~1:500 FC: 1:100~1:200

Background :Actin proteins are major components of the eukaryotic cytoskeleton. At least six vertebrate actin isoforms have been identified. The cytoplasmic β- and γ-actin proteins are referred to as “non-muscle” actin proteins as they are predominantly expressed in non-muscle cells where they control cell structure and motility. The α-cardiac and α-skeletal actin proteins are expressed in striated cardiac and skeletal muscles, respectively. The smooth muscle α-actin and γ-actin proteins are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. The α-smooth muscle actin (ACTA2) is also known as aortic smooth muscle actin. These actin isoforms regulate the contractile potential of muscle cells .

Product :Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :alpha Smooth Muscle Actin monoclonal antibody detects endogenous levels of alpha Smooth Muscle Actin protein.

BiowMW :~ 43 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACTSA; ACTVS; Actin, aortIC, smooth muscle; Alpha-actin-2; Cell growth-inhibiting gene 46 protein

Immunogen :Recombinant fusion protein of human alpha Smooth Muscle Actin expressed in E. Coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of alpha Smooth Muscle Actin expression in EC (A), HUVE12 (B), A549 (C), NIH/3T3 (D), HL60 (E), Hela (F), K652 (G) whole cell lysates.

Immunohistochemical analysis of alpha Smooth Muscle Actin staining in human bladder cancer formalin fixed paraffin embedded tissue section.

Flow cytometric analysis of HeLa cells using Anti-alpha Smooth Muscle Actin Antibody (green) and negative control (red).