Western blot analysis of Histone H3 (TriMethyl K9) expression in Hela (A) whole cell lysates.
Immunohistochemical analysis of Histone H3 (TriMethyl K9) staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemistry of paraffin-embedded human lung cancer using PPP4C antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
