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PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody (KU)

货号: MB4153

价格：￥ 398

货期：现货（1-2个工作日）

25ul 50ul 100ul

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No. :MB4153

Product Name :PRKAR1A (PKA regulatory subunit I alpha) mouse monoclonal antibody (KU)

Swiss-Prot :P10644

Host :Mouse

Reactivity :Human, Mouse, Rat

Applications :IF, IHC, WB

Application_all :WB 1:200, IHC 1:150, IF 1:100

Background :cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites fluids or tissue culture supernatant by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG1

BiowMW :~ 53 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :E3 ubiquitin-protein ligase TRIM38; EC:2.3.2.27; RING finger protein 15; Tripartite motif-containing protein 38 ; Zinc finger protein RoRet; TRIM38

Immunogen :Human recombinant protein fragment corresponding to amino acids 1-265 of human TRIM38(NP_006346) produced in E.coli.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of TRIM38 mouse monoclonal antibody at 1:500 dilution. Lane1:HepG2 whole cell lysate(30ug)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIM38 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Anti-TRIM38 mouse monoclonal antibodyimmunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TRIM38 (RC201944).