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NCF4 monoclonal antibody

货号: MB12176

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB12176

Product Name :NCF4 monoclonal antibody

Swiss-Prot :Q15080

Host :Rabbit

Reactivity :Human

Applications :WB,IHC-F,IHC-P,ICC/IF,IP

Application_all :WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20

Background :The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product :50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Purification&Purity :Affinity Purified

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :Calculated MW: 39 kDa; Observed MW: 39 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NCF; CGD3; P40PHOX; SH3PXD4

Immunogen :A synthetic peptide of human p40-phox

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of p40phox in HL-60, U251 lysates using NCF4 antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using p40phox antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.