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Phospho-Nucleophosmin (Thr199) monoclonal antibody

货号: MB11879

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB11879

Product Name :Phospho-Nucleophosmin (Thr199) monoclonal antibody

Swiss-Prot :P06748

Host :Rabbit

Reactivity :Human,Rat

Applications :WB,IHC-P,IP

Application_all :WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background :Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).

Product :50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Purification&Purity :Affinity Purified

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :Calculated MW: 33 kDa; Observed MW: 38 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :B23; NPM

Immunogen :A synthetic phosphopeptide corresponding to residues surrounding Thr199 of human Nucleophosmin

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of Nucleophosmin in C6, Ramos lysates using Phospho-Nucleophosmin antibody.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Nucleophosmin antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.