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PHD3 monoclonal antibody

货号: MB11408

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB11408

Product Name :PHD3 monoclonal antibody

Swiss-Prot :Q9H6Z9

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB,IHC-P,IP

Application_all :WB: 1/500-1/1000 IHC: 1/50-1/100 IP: 1/20

Background :Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Also hydroxylates PKM in hypoxia, limiting glycolysis. Under normoxia, hydroxylates and regulates the stability of ADRB2. Regulator of cardiomyocyte and neuronal apoptosis. In cardiomyocytes, inhibits the anti-apoptotic effect of BCL2 by disrupting the BAX-BCL2 complex. In neurons, has a NGF-induced proapoptotic effect, probably through regulating CASP3 activity. Also essential for hypoxic regulation of neutrophilic inflammation. Plays a crucial role in DNA damage response (DDR) by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway. Target proteins are preferentially recognized via a LXXLAP motif.

Product :50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Purification&Purity :Affinity Purified

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :Calculated MW: 27 kDa; Observed MW: 27 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PHD3; HIFPH3; HIFP4H3

Immunogen :Recombinant protein of human PHD3

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of PHD3 in Hela lysates using PHD3 antibody.

Western blot analysis of PHD3 in rat lung and mouse lung lysates using PHD3 antibody.