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Histone H2A.X (phospho-S139) monoclonal antibody

货号: MB0175

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0175

Product Name :Histone H2A.X (phospho-S139) monoclonal antibody

Swiss-Prot :P16104

Host :Mouse

Reactivity :Human,Mouse

Applications :WB IF

Application_all :WB: 1:1000~2000 IF: 1:200~500

Background :H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobulin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139, and does not cross-react with related proteins.

BiowMW :Predicted band size:15KDa Observed band size:15KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Histone H2AX; H2a/x; Histone H2A.X; H2AFX; H2AX; γ-H2AX; γH2AX; ganunaH2AX; γ-H2A.X; γH2A.X; ganunaH2A.X

Immunogen :Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in A549(upper,untreated or Hydroxyurea-treated) and Hela(lower,untreated or Hydroxyurea-treated) using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

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Journal10.1016/j.cellsig.2024.111451

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PMID39389178