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BID monoclonal antibody

货号: MB0118

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :MB0118

Product Name :BID monoclonal antibody

Swiss-Prot :P55957

Host :Mouse

Reactivity :Human

Applications :WB ICC

Application_all :WB: 1:300~1000 ICC: 1:100

Background :BID, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BID mAb detects endogenous levels of BID protein.

BiowMW :Predicted band size:22KDa Observed band size:22KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Inhibition of Apoptosis,Mitochondrial Control of Apoptosis,Death Receptor Signaling,

Alternative Name :BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID;,BH3-interacting domain death agonist p11; p11 BID; BID

Immunogen :Recombinant full length Human BID.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of BID antibody in 293T, Jurkat and A431 cell lysates using BID antibody (1:300 diluted) .

Immunocytochemistry staining of HeLa cells using anti-BID antibody (dilution 1:100).