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HSP70 (N-terminus) monoclonal antibody

货号: MB0105

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :MB0105

Product Name :HSP70 (N-terminus) monoclonal antibody

Swiss-Prot :P08107

Host :Mouse

Reactivity :Human

Applications :WB ICC

Application_all :WB: 1:1000 ICC: 1:100~300

Background :The HSP 70 family is composed of four highly conserved proteins: HSP 70, HSC 70, GRP 75 and GRP 78. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70 play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery and are found in both the cytosol and nucleus of mammalian cells. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. It has been postulated that members of the HSP 70 family act as force-generating motors, relying on the hydrolysis of ATP for their activity

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of Hsp70 and does not cross-react with related proteins

BiowMW :Predicted band size:70KDa Observed band size:70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2; HSPA1A; HSPA1; HSX70; HSPA1B; HSP-70; HSP 70

Immunogen :Synthetic peptide, corresponding to the N-terminus of Human HSP70.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of Hsp70 (N-terminus) antibody in Heat shcok Hela, Hela&HepG2 lysates using Hsp70 (N-terminus) antibody (1:1000 diluted) .

ICC staining delta 1 Catenin/CAS in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with delta 1 Catenin/CAS polyclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).

Heat stress inhibits proliferation, promotes growth, and induces apoptosis in cultured Lantang swine skeletal muscle satellite cells

JournalJournal of Zhejiang University-SCIENCE B

ApplicationWB

IF1.879

SpeciesPig

PMID26055917