产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ATP-Citrate Lyase (C-terminus) monoclonal antibody

货号: MB0070

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :MB0070

Product Name :ATP-Citrate Lyase (C-terminus) monoclonal antibody

Swiss-Prot :P53396

Host :Mouse

Reactivity :Human

Applications :WB ICC FC

Application_all :WB: 1:1000 ICC: 1:50~200 FC: 1:50~100

Background :ATP citrate lyase (ACL) is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of four identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. One of these products, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. NDPK has been found to phosphorylate ACL and insulin to increase phosphorylation of ACL.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :This antibody detects endogenous levels of ATP-Citrate Lyase (C-terminus) and does not cross-react with related proteins

BiowMW :Predicted band size:120KDa Observed band size:120KDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Warburg Effect,Glutamine Metabolism,AMPK Signaling Pathway,

Alternative Name :ATP-citrate synthase; ATP-citrate (pro-S-)-lyase; ACL; Citrate cleavage enzyme; ACLY

Immunogen :Synthetic peptide, corresponding to amino acids C-terminus of Human ATP-Citrate Lyase.

Conjugate :Unconjugated

Modification :Unmodification

Western blot detection of ATP-Citrate Lyase antibody in K562&Hela cell lysates using ATP-Citrate Lyase antibody (1:1000 diluted) .

Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG

Immunocytochemistry of HeLa cells using anti- ATP-Citrate Lyase (C-terminus) antibody diluted 1:150.