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M-RIP polyclonal antibody（KU）

货号: CAS7516

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :CAS7516

Product Name :M-RIP polyclonal antibody（KU）

Swiss-Prot :Q6WCQ1

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IHC

Application_all :WB: 1:5000~1:10000 IP:1:2000~1:8000 IHC:1:50~1:100

Background :Myosin phosphatase-rho interacting protein (M-RIP), also known as p116RIP, RIP3, and MPRIP, localizes to actin-myosin filaments regulating cytoskeletal dynamics. M-RIP contains amino-terminal pleckstrin homology domains, carboxyl-terminal coiled-coil domains, and was originally identified to associate with the myosin phosphatase complex. M-RIP binds to MBS/MYRT, the myosin binding subunit of myosin phosphatase, as well as RhoA. Phosphorylation of MYRT by Rho-associated kinase (ROCK) inhibits myosin phosphatase activity, resulting in increased levels of phosphorylation on myosin light chain, and enhanced contractility. M-RIP may function as a scaffolding protein for the complex between the myosin phosphatase complex, Rho/ROCK, and actin. Silencing of M-RIP results in disassembly of the complex, increased phosphorylation of myosin light chain, and changes to cytoskeletal dynamics.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :M-RIP polyclonal antibody detects endogenous levels of M-RIP protein.

BiowMW :~ 130-160kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Myosin phosphatase Rho-interacting protein; M-RIP;Rho-interacting protein 3;RIP3;p116Rip;MPRIP

Immunogen :Recombinant protein of human M-RIP.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of M-RIP polyclonal antibody at 1:5000 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:Myla2059 whole cell lysate(40ug) Lane5:PC3 whole cell lysate(40ug) Lane6:SGC7901 whole cell lysate(40ug)

Immunoprecipitation - M-RIP Polyclonal Antibody

Immunohistochemistry of paraffin-embedded human colon carcinoma using M-RIP antibody at dilution of 1:50.