产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Phospho-Artemis (Ser516) polyclonal antibody

货号: BZ16478

价格：￥ 1350

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BZ16478

Product Name :Phospho-Artemis (Ser516) polyclonal antibody

Swiss-Prot :Q96SD1

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB,IHC-P,ELISA

Application_all :WB: 1/500-1/1000 IHC: 1/50-1/100 ELISA: 1/10000

Background :Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :Affinity Purified

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :Calculated MW: 78 kDa; Observed MW: 78 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SCIDA; SNM1C; A-SCID; RS-SCID; DCLREC1C

Immunogen :The antiserum was produced against synthesized peptide derived from human Artemis around the phosphorylation site of Ser516. AA range:482-531

Conjugate :Unconjugated

Modification :Phosphorylated

Immunohistochemistry analysis of paraffin-embedded Human lung carcinoma, using Phospho-Artemis antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.

Western blot analysis of Phospho-Artemis in HepG2 cells treated with EGF , using Phospho-Artemis antibody.The lane on the right is blocked with the Phospho- peptide.