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Phospho-PKA alpha/beta/gamma (Thr197) polyclonal antibody

货号: BZ16474

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BZ16474

Product Name :Phospho-PKA alpha/beta/gamma (Thr197) polyclonal antibody

Swiss-Prot :P17612/P22694/P22612

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB,IHC-F,IHC-P,ICC/IF,ELISA

Application_all :WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background :PRKACA (protein kinase cAMP-activated catalytic subunit alpha) encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms.

Product :Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Purification&Purity :Affinity Purified

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IgG

BiowMW :Calculated MW: 40 kDa; Observed MW: 40 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PRKACA; PKACA; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; PRKACB; cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACG; cAMP-dependent protein kinase catalytic subunit gamma; PKA C-gamma

Immunogen :The antiserum was produced against synthesized peptide derived from human PKA CAT around the phosphorylation site of Thr197. AA range:166-215

Conjugate :Unconjugated

Modification :Phosphorylated

EnzymeLinked Immunosorbent Assay for Immunogen Phospho-peptide and NonPhospho-peptide , using PKA CAT (Phospho-Thr19antibody

Immunofluorescence analysis of Phospho-PKA alpha/beta/gamma in A549 using Phospho-PKA alpha/beta/gamma antibody. The picture on the right is blocked with the Phospho- peptide.

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-PKA alpha/beta/gamma antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Sample with blocking peptide on the right.