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BUB1 (F818) polyclonal antibody（KU）

货号: BS9180

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS9180

Product Name :BUB1 (F818) polyclonal antibody（KU）

Swiss-Prot :O43683

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Human cells contain two related protein kinases, BUB1 and BUBR1, that appear to have evolved from a single ancestral BUB1 gene. Both kinases are concentrated near the surface of the kinetochore where they monitor kinetochore-microtubule interactions. BUB1 and BUBR1 bind to kinetochores and are postulated to be components of the mitotic checkpoint, which monitors kinetochore activities to determine if chromosomes have achieved alignment at the spindle equator. BUBR1 is essential for normal mitotic progression as it prevents cells from prematurely entering anaphase. BUB3 is a conserved component of the mitotic spindle assembly complex and is also involved with the essential spindle checkpoint pathway that operates during early embryogenesis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :BUB1 (F818) polyclonal antibody detects endogenous levels of BUB1 protein.

BiowMW :~ 122 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A; BUB1; BUB1L

Immunogen :Synthetic peptide, corresponding to amino acids 791-840 of Human BUB1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of BUB1 (F818) pAb at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:HuT78 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug)