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PRTN3 polyclonal antibody

货号: BS91120

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS91120

Product Name :PRTN3 polyclonal antibody

Swiss-Prot :P24158

Host :Rabbit

Reactivity :Human

Applications :WB ELISA IHC IF ICC

Application_all :WB: 1:500-1:2000 ELISA: 1:5000-1:10000 IHC: 1:100-1:500 IF: 1:100-1:500 ICC: 1:100-1:500

Background :The major features of Wegener granulomatosis are necrotizing granulomatous lesions, which most often affect the upper and lower airways and are associated with vasculitis, necrotizing glomerulonephritis and pulmonary capillaritis. The antigen responsible for this disease is Proteinase 3 (PR3, P29 or myeloblastin), which is one of the antibiotic proteins of neutrophilic granules belonging to the serine protease family. It is closely related to two others: Neutrophil Elastase and azurocidin. All three genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. PR3 is a neutrophil protein which is able to cleave elastin and is involved in proliferation of human leukemia cells. PR3 is expressed specifically in immature myeloid cells and is a G-CSF-responsive protein critical to factor-independent growth. The genes for all three of the related serine protease family members are located in a cluster on the tip of the short arm of human chromosome 19.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity :PRTN3 polyclonal antibody detects endogenous levels of PRTN3 protein.

BiowMW :~ 28 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACPA; AGP 7; AGP7; AGP7 serine proteinase; Azurophil Granule Protein 7; C ANCA antigen; C-ANCA antigen; EC 3.4.21.76; Leukocyte proteinase 3; MBN; MBT WEGENER AUTOANTIGEN; Myeloblastin; Neutrophil proteinase 4; NP 4; NP-4; NP4; P29; PR 3; PR-3; PR3; Proteinase3; PRTN 3; Prtn3; PRTN3_HUMAN; Serine proteinase neutrophil Wegener granulomatosis autoantigen; Wegener autoantigen

Immunogen :KLH conjugated synthetic peptide derived from human PR3: 21-120/256

Conjugate :Unconjugated

Modification :Unmodification

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-PR3 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C.

Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-PR3 Polyclonal Antibody, Unconjugated 1:500, overnight at 4°C.