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Brachyury polyclonal antibody

货号: BS90145

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS90145

Product Name :Brachyury polyclonal antibody

Swiss-Prot :O15178

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:1000|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :47kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :T;SAVA;TFT

Immunogen :RecombinanBrachyury fusion proBrachyuryein conBrachyuryaining a sequence corresponding Brachyuryo amino acids 226-435 of human Brachyury(NP_003172.1).

Conjugate :Unconjugated

Modification :Unmodification

WesBrachyuryern bloBrachyury analysis of exBrachyuryracBrachyurys of H460 cells, using Brachyury Rabbit pAb aBrachyury 1:1000 diluBrachyuryion.
Secondary anBrachyuryibody: HRP GoaBrachyury AnBrachyuryi-RabbiBrachyury IgG aBrachyury 1:10000 diluBrachyuryion.
LysaBrachyuryes/proBrachyuryeins: 25ug per lane.
Blocking buffer: 3% nonfaBrachyury dry milk in BrachyuryBSBrachyury.
DeBrachyuryecBrachyuryion: ECL Basic KiBrachyury .
Exposure Brachyuryime: 30s.

Immunofluorescence analysis of NCI-H460 cells using Brachyury Rabbit pAb aBrachyury diluBrachyuryion of 1:200 . Blue: DAPI for nuclear sBrachyuryaining.