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α-Smooth Muscle Actin (ACTA2) polyclonal antibody

货号: BS8796

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8796

Product Name :α-Smooth Muscle Actin (ACTA2) polyclonal antibody

Swiss-Prot :P62736

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100|IF/ICC,1:100 - 1:200

Background :The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :42KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AAT6;ACTSA;MYMY5;ACTA2;actin

Immunogen :Recombinant fusion protein of human α-Smooth Muscle Actin (ACTA2)(NP_001135417.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Mouse heart, using α-Smooth Muscle Actin antibody at 1:810 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using α-Smooth Muscle Actin Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophagus using α-Smooth Muscle Actin Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded Human tonsil using α-Smooth Muscle Actin Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Participation of CCL1 in Snail-Positive Fibroblasts in Colorectal Cancer Contribute to 5-Fluorouracil/Paclitaxel Chemoresistance

JournalCancer Research and Treatment

ApplicationIHC

IF3.761

SpeciesHuman

PMID28934847
Macrophages activate mesenchymal stem cells to acquire cancer‑associated fibroblast‑like features resulting in gastric epithelial cell lesions and malignant transformation in vitro

JournalOncology Letters

ApplicationWB

IF2.311

SpeciesHuman

PMID30655826