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EIF3H polyclonal antibody（KU）

货号: BS8655

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8655

Product Name :EIF3H polyclonal antibody（KU）

Swiss-Prot :O15372

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB IHC IP IF

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200|IF,1:50 - 1:200

Background :Component of the eukaryotic translation initiation factor 3 (eIF-3 complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC. The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :EIF3H polyclonal antibody detects endogenous levels of EIF3H protein.

BiowMW :40kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :EIF3H;EIF3S3;eIF3-gamma;eIF3-p40

Immunogen :Recombinant fusion protein of human EIF3H(NP_003747.1).

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of EIF3H pAb at 1:5000 dilution Lane1:EC9706 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug) Lane4:The Pancreas tissue lysate of Mouse(40ug) Lane5:The Thymus tissue lysate of Rat(40ug) Lane6:PC12 whole cell lysate(40ug) Lane7:RAW264.7 whole cell lysate(40ug)

Immunohistochemical analysis of EIF3H staining in human colorectal cancer formalin fixed paraffin embedded tissue section.

Immunofluorescence analysis of PC3 cells using EIF3H pAb at dilution of 1:50.

Immunoprecipitation of Jurkat cell lysates using EIF3H pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 1 is 30% input. The western blot was probed using EIF3H pAb.