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POLR2H polyclonal antibody

货号: BS8603

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS8603

Product Name :POLR2H polyclonal antibody

Swiss-Prot :P52434

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IP

Application_all :WB,1:500 - 1:2000|IP,1:50 - 1:200

Background :The three eukaryotic RNA polymerases are complex multisubunit enzymes that play a central role in the transcription of nuclear genes. This gene encodes an essential and highly conserved subunit of RNA polymerase II that is shared by the other two eukaryotic DNA-directed RNA polymerases, I and III. Alternative splicing results in multiple transcript variants of this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :17kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :POLR2H;RPABC3;RPB17;RPB8

Immunogen :Recombinant fusion protein of human POLR2H(NP_006223.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using POLR2H antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunoprecipitation analysis of 200ug extracts of MCF7 cells using 1ug POLR2H antibody . Western blot was performed from the immunoprecipitate using POLR2H antibody at a dilution of 1:1000.