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Survivin polyclonal antibody

货号: BS8456

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS8456

Product Name :Survivin polyclonal antibody

Swiss-Prot :O15392

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :16KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :API4;EPR-1;BIRC5;Survivin

Immunogen :Recombinant fusion protein of human Survivin(NP_001159.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using Survivin antibody at 1:454 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded Human brain using Survivin Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human colon carcinoma using Survivin Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophageal cancer using Survivin Rabbit pAb at dilution of 1:25 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Loss of interactions between p53 and survivin gene in mesenchymal stem cells after spontaneous transformation in vitro

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB

IF3.144

SpeciesRat

PMID27046449
Perichondrium mesenchymal stem cells inhibit the growth of breast cancer cells via the DKK-1/Wnt/β-catenin signaling pathway

JournalOncology Reports

ApplicationWB

IF3.041

SpeciesRat

PMID27277008
miR-31 promotes proliferation of colon cancer cells by targeting E2F2

JournalBiotechnology Letters

ApplicationWB

IF2.461

SpeciesHuman

PMID25362258
Effects of infrasound on the growth of bone marrow mesenchymal stem cells: A pilot study

JournalMolecular Medicine Reports

ApplicationIHC

IF1.851

SpeciesRat

PMID25175368
RASSF10 suppresses colorectal cancer growth by activating P53 signaling and sensitizes colorectal cancer cell to docetaxel

JournalOncotarget

ApplicationWB

IF0

SpeciesHuman

PMID25638156