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PPP1R12A polyclonal antibody

货号: BS8367
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS8367
Product Name :PPP1R12A polyclonal antibody
Swiss-Prot :O14974
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200
Background :Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :130kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :PPP1R12A;M130;MBS;MYPT1;MYPT1
Immunogen :Recombinant fusion protein of human PPP1R12A(NP_002471.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using PPP1R12A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse heart using PPP1R12A Antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Inhibition of Rho‑kinase is involved in the therapeutic effects of atorvastatin in heart ischemia/reperfusion
    JournalExperimental and Therapeutic Medicine
    ApplicationWB
    IF1.785
    SpeciesRat
  • Opticin Ameliorates Hypoxia-Induced Retinal Angiogenesis by Suppression of Integrin α2-I Domain–Collagen Complex Formation and RhoA/ROCK1 Signaling
    JournalInvestigative Ophthalmology & Visual Science
    ApplicationWB
    IF4.799
    SpeciesZebrafish

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com