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PPP1R12A polyclonal antibody

货号: BS8367

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8367

Product Name :PPP1R12A polyclonal antibody

Swiss-Prot :O14974

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :130kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PPP1R12A;M130;MBS;MYPT1;MYPT1

Immunogen :Recombinant fusion protein of human PPP1R12A(NP_002471.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using PPP1R12A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded mouse heart using PPP1R12A Antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Involvement of RhoA/ROCK in myocardial fibrosis in a rat model of type 2 diabetes

JournalActa Pharmacologica Sinica

ApplicationWB

IF5.064

Species

PMID21743486
ERK-MAPK Signaling Opposes Rho-Kinase to Reduce Cardiomyocyte Apoptosis in Heart Ischemic Preconditioning

JournalMolecular Medicine

ApplicationWB

IF2.991

SpeciesRat

PMID20383434
Fasudil hydrochloride hydrate, a Rho-kinase inhibitor, ameliorates hepatic fibrosis in rats with type 2 diabetes

JournalChinese Medical Journal

ApplicationWB

IF2.628

SpeciesRat

PMID
Fasudil hydrochloride hydrate, a Rho-kinase inhibitor, suppresses high glucose-induced proliferation and collagen synthesis in rat cardiac fibroblasts

JournalClinical And Experimental Pharmacology And Physiology

ApplicationWB

IF2.557

SpeciesRat

PMID21457293
Simvastatin exerts cardioprotective effects and inhibits the activity of Rho‐associated protein kinase in rats with metabolic syndrome

JournalClinical And Experimental Pharmacology And Physiology

ApplicationWB

IF2.456

SpeciesRat

PMID22670687