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LIMS1 polyclonal antibody

货号: BS8174

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS8174

Product Name :LIMS1 polyclonal antibody

Swiss-Prot :P48059

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:100

Background :The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :40kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LIMS1;PINCH;PINCH-1;PINCH1

Immunogen :Recombinant fusion protein of human LIMS1(NP_001180417.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HepG2 cells, using LIMS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Immunofluorescence analysis of MCF7 cells using LIMS1 antibody . Blue: DAPI for nuclear staining.