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SMAD2/SMAD3 polyclonal antibody

货号: BS80508

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS80508

Product Name :SMAD2/SMAD3 polyclonal antibody

Swiss-Prot :P84022/Q15796

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB:1:500 - 1:1000 IHC:1:50 - 1:200 IF/ICC:1:50 - 1:200

Background :The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SMAD2/SMAD3 polyclonal antibody detects endogenous levels of SMAD2/SMAD3 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HSPC193;HsT17436;JV15-2;LDS1C;LDS3;MADH3

Immunogen :A synthetic peptide corresponding to a sequence within amino acids 150-250 of human SMAD2/SMAD3 (NP_005892.1).

Conjugate :Unconjugated

Modification :Unmodified

Western blot analysis of extracts of various cell lines, using SMAD2/SMAD3 antibody at 1:700 dilution.

Immunohistochemistry analysis of paraffin-embedded human kidney using SMAD2/SMAD3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human gastric using SMAD2/SMAD3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using SMAD2/SMAD3 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.