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[KO Validated] Fatty Acid Synthase (FASN) polyclonal antibody

货号: BS80301

价格：￥ 1540

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS80301

Product Name :[KO Validated] Fatty Acid Synthase (FASN) polyclonal antibody

Swiss-Prot :P49327

Host :Rabbit

Reactivity :Human, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :273KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :FASN; FAS; OA-519; SDR27X1; fatty acid synthase

Immunogen :Recombinant fusion protein of human Fatty Acid Synthase (FASN)(NP_004095.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using Fatty Acid Synthase Rabbit pAb antibody at 1:1330 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 0.5s.