产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

[KO Validated] PARP1 polyclonal antibody

货号: BS80292

价格：￥ 1540

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS80292

Product Name :[KO Validated] PARP1 polyclonal antibody

Swiss-Prot :P09874

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200

Background :This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :114kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PARP1; ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP; PARP-1; PPOL; pADPRT-1; poly(ADP-ribose) polymerase 1

Immunogen :A synthetic peptide of human PARP1(NP_001609.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from wild type and PARP1 knockout HeLa cells, using PARP1 antibody at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] PARP1 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.