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FADD polyclonal antibody

货号: BS80252

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS80252

Product Name :FADD polyclonal antibody

Swiss-Prot :Q61160

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:200 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95 or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :28KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Mort1/F; Mort1/FADD

Immunogen :Recombinant fusion protein of mouse FADD(NP_034305.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse heart, using FADD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Western blot analysis of extracts of Jurkat cells, using FADD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.