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SARS-CoV-2 Spike S1 polyclonal antibody

货号: BS80242

价格：￥ 1460

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS80242

Product Name :SARS-CoV-2 Spike S1 polyclonal antibody

Swiss-Prot :P0DTC2

Host :Rabbit

Reactivity :SARS-CoV-2

Applications :DB, WB, IF/ICC, IP

Application_all :DB,1:500 - 1:2000|ELISA,1:20000-1:80000|WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :110KDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Recombinant fusion protein coronavirus Spike S1(YP_009724390.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of 293T cells, using SARS-CoV-2 Spike S1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immobilized Recombinant SARS-COV-2 Spike S1 Protein at 1μg/mL can bind SARS-CoV-2 Spike S1 Rabbit pAb with a linear range of 0.78-50ng/mL.