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SARS-CoV-2 Nucleoprotein polyclonal antibody

货号: BS80224

价格：￥ 1460

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

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No. :BS80224

Product Name :SARS-CoV-2 Nucleoprotein polyclonal antibody

Host :Rabbit

Reactivity :SARS-CoV-2

Applications :WB, IF/ICC, DB, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|DB,1:500 - 1:2000|IP,1:50 - 1:200

Background :Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :50kDa

Note :For research use only, not for use in diagnostic procedure.

Immunogen :Recombinant fusion protein of coronavirus Nucleoprotein(P0DTC9).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of normal 293T cells and 293T transfected with Nucleoprotein,using SARS-CoV-2 Nucleoprotein antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of Recombinant Nucleoprotein of Coronavirus SARS-CoV-2，using SARS-CoV-2 Nucleoprotein antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.