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IκBα polyclonal antibody

货号: BS80104

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS80104

Product Name :IκBα polyclonal antibody

Swiss-Prot :P25963

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :36kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IKBA;MAD-3;NFKBI;IKB alpha;NFKBIA;EDAID2

Immunogen :A synthetic peptide of human IκBα(NP_065390.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using IκBα antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Immunofluorescence analysis of NIH/3T3 cells using IκBα Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using IκBα Rabbit pAb at dilution of 1:250 . Blue: DAPI for nuclear staining.