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Bcl-2 polyclonal antibody

货号: BS80057

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS80057

Product Name :Bcl-2 polyclonal antibody

Swiss-Prot :P10415

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Unmodification

BiowMW :26kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Bcl-2;PPP1R50;BCL2

Immunogen :Recombinant fusion protein of human Bcl-2(P10415).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse lung, using Bcl-2 Rabbit pAb at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 5s.

Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 antibody at dilution of 1:250. Blue: DAPI for nuclear staining.

Therapeutic effect of notoginseng saponins before and after fermentation on blood deficiency rats

JournalExperimental and Therapeutic Medicine

ApplicationWB

IF2.7

SpeciesRat

PMID38476921